2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

CD157

The CD157 antigen, also known as bone marrow stromal cell antigen 1 (BST-1) is a highly glycosylated, glycosyl-phosphatidylinositol (GPI)-anchored membrane protein with a molecular weight of 43 kDa. The amino acid sequence of human BST-1 has 33% identity with CD38. Both CD38 and CD157 play dual roles as receptors and ectoenzymes, endowed with complex activities related to signaling and cell homeostasis. As with CD38, BST-1 displays ADP ribosyl cyclase activity and facilitates pre-B cell growth. CD157 is constitutively expressed by myeloid cells in peripheral blood mononuclear cells (PBMCs). The molecule is also expressed by synovial, vascular endothelial and follicular dendritic cells. Moreover, CD157 is also present on other cell types and tissues, such as dermal fibroblasts, human mast cells from lung, uterus, foreskin, and peritoneal mesothelial cells, among others.

Lysed normal whole blood sample.

Clone: RF3 (IgG Mouse) TheRF3antibodyreactswithan externalepitopeof BST-1on severalhumancelllinesincludingcertainbonemarrowstromalcell,humanumbilicalveinendothelial cell (HUVEC) and U937 cell lines.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC

IM2557

0.05 mg

Liquid

RUO

-

cGMP

Clone: SY/11B5 (IgG1 Mouse)

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

B68160

1 mL

Liquid

ASR

IOTest

cGMP

CD158a,h

The CD158a and CD158h molecules are monomeric integral membrane glycoproteins existing in different forms. CD158a, or p58.1, is a 58 kDa molecule, and CD158h, or p50.1, is a 50 kDa molecule, both having identical extracellular portions, but different transmembrane and cytoplasmic regions. Another comprehensive nomenclature refers to these molecules as KIR2DL1 for CD158a and KIR2DS1 for CD158h. These acronyms stand for Killer cell Immunoglobulin (Ig)-like Receptor (KIR), with extracellular part containing 2 Ig Domains (2D), and cytoplasmic tails being either long (L) or small (S). The long intracellular portion of CD158a, or p58.1, makes it an inhibitory form, since it contains two characteristic ITIM motifs (Immunoreceptor tyrosine based inhibition motif) and is involved in the transduction of the inhibitory signal. Whereas the stimulatory form CD158h, or p50.1, lacks these ITIM motifs. CD158a/p58.1 functions as an inhibitory receptor for a group of HLA-C molecules (Cw2, cw4, Cw5 and Cw6 supertypes). The inhibitory effect is dominant over the activation exerted by the activator form p50.1. The CD158a and CD158h molecules are indistinguishable from the extracellular viewpoint. They are expressed by a subset of NK cells, and can be detected on the surface of sub-populations of peripheral T lymphocytes, mostly of the CD8+ phenotype.

Lysed normal whole blood sample.

Clone: EB6B (IgG1 Mouse) TheEB6BantibodyreactswithbothCD158a(p58.1or KIR2DL1)andCD158h(p50.1or KIR2DS1).Theantibodyrestorestheabilityof p58.1

+ NKclonesto lysetargets

expressing HLA-C molecules.

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

A09778 A66898 A66899 A22332

1 mL

Liquid Liquid Liquid Liquid

ASR ASR ASR ASR

IOTest IOTest IOTest IOTest

cGMP cGMP cGMP cGMP

PC5.5

0.5 mL 0.5 mL 0.5 mL

PC7 APC

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 92 Outside US Flow Cytometry Catalog