2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

CD94 is a type II transmembrane glycoprotein of 30 kDa, belonging to the Ca++- dependent (C-type) lectin family. Like NKG2A (or Kp43) on human natural killer (NK) cells, CD94 associates with one member of the NKG2 family to form disulphide-linked, NK cell receptor for MHC class I molecules, with a broader specificity than the human killer cell inhibitory (KIR) / activator (KAR) receptors of the Ig-superfamily. Ligation of CD94 either potently triggers or inhibits NK cell proliferation and cell-mediated cytotoxicity. Recent studies strongly indicate that the specificity of CD94/NKG2 receptors is for HLA-E, a non-classical MHC class I molecule. CD94/NKG2A and CD94/NKG2B heterodimers constitute inhibitory NK cell receptors, whereas the association of CD94 with NKG2C corresponds to an activating receptor. The expression of CD94 appears restricted to most NK cells and to a T lymphocyte subpopulation including a subset of γδ TCR+ T-cells and αβ TCR+ CD8+ CD56+ T- cells, mainly V α 2/V δ 2. CD94 ( NKG2a )

Lysed normal whole blood sample.

Clone: HP-3B1 (IgG2a Mouse) Ligationof CD94withthe HP-3B1monoclonalantibodyinhibitsIL-2-dependentproliferationof NK-cells,and inducesapoptosisin a subsetof IL-2-stimulatedNK cells.

Form

Format

Part#

Status

Line

Quality Standard

Size

PB PE

B90465 IM2276 B09980

0.5 mL

Liquid Liquid Liquid

ASR RUO ASR

IOTest IOTest IOTest

cGMP cGMP cGMP

100 tests

APC

0.5 mL

CD95

The CD95 (Fas or APO-1) antigen is a 40-50 kDa transmembrane glycoprotein. It belongs to the Tumor Necrosis Factor (TNF) superfamily and contains three cysteine-rich repeats. This cell surface molecule mediates apoptosis (programmed cell death). CD95 antigen is expressed on a substantial proportion of peripheral blood CD4+ lymphocytes, CD8+ lymphocytes and B lymphocytes but on a minor proportion of NK cells. It is not found on platelets or erythrocytes, but is expressed variably on granulocytes and monocytes. CD95 is strongly up-regulated on activated T cells, B cells, NK cells and thymocytes. Moreover, it is widely expressed on cell lines of T, B, NK and myeloid lineages. Most other tissues express CD95.

Clone: CH11 (IgM Mouse) The CH11 antibody in vitro induces apoptosis in some CD95 expressing cell lines.

Form

Format

Part#

Status

Line

Quality Standard

Size

UNLB

IM1504

0.05 mg

Liquid

RUO

-

cGMP

Clone: UB2 (IgG1 Mouse) UB2 cross reacts with: Carp.

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

IM1739

50 tests

Liquid

RUO

-

cGMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 80 Outside US Flow Cytometry Catalog