2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

CD86

The CD86 antigen (B7-2, B70) is a single-chain trans-membrane glycoprotein, structurally similar to CD80. Its molecular weight is 80 kDa, under reducing conditions. The extracellular region is composed of one V-type and one C-type Ig-like domains. There are 8 potential sites for N-glycosylation. The cytoplasmic tail has 3 potential sites for protein kinase C phosphorylation. CD86 shares with the CD80 molecule the same co-receptors on T cells, CD28 and CD152 (CTLA-4). CD86 binds to CD152 with a 20-100-fold higher affinity than to CD28. CD86 is expressed constitutively by interdigitating dendritic cells, and at lower level by peripheral blood dendritic cells. It is strongly expressed by monocytes. On lymphocytes, CD86 appears as a B-cell activation antigen. CD86 is preferentially expressed by memory B cells and germinal center B cells, but not on plasma cells. Its expression can be up-regulated by activation through surface immunoglobulin, CD40, MHC class II molecules or by PMA with ionomycin. T cells activated by CD3 ligation have also been reported to express CD86.

Lysed normal whole blood sample.

Clone: HA5.2B7 (IgG2b Mouse) HA5.2B7 cross reacts with: Ovine, Caprine, Porcine, Mink.

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

IM2729U B30647 B30648 B30646

2 mL

Liquid Liquid Liquid Liquid

ASR ASR ASR ASR

IOTest IOTest IOTest IOTest

cGMP cGMP cGMP cGMP

PC5.5

0.5 mL 0.5 mL 0.5 mL

PC7

APC-A750

CD90

The human CD90 antigen (also known as Thy-1) is the smallest (18 kDa) member of the immunoglobulin gene superfamily (IgSF). It is a highly glycosylated molecule with a glycosyl-phosphatidylinositol (GPI) anchor consisting of a single IgSF variable domain. The CD90 molecule has a broad tissue distribution and is expressed in nervous tissue, connective tissue and various stromal cell lines. It characterizes a rare subset of human fetal bone marrow cells, that contains multipotent hematopoietic progenitor activity. This antigen is expressed on a subset of CD34 positive cells from human bone marrow, cord blood or fetal liver. It is also expressed on very small subsets of thymocytes and peripheral T-lymphocytes.

Jurkat cell line: IL-2 producing T lymphocyte cell line.

Clone: F15-42-1-5 (IgG1 Mouse) F15-42-1-5 cross reacts with: Cynomolgus Monkey, Indian Rhesus, Hamadyras Baboon, Olive Baboon.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC

IM1839U IM1840U

2 mL 2 mL

Liquid Liquid

ASR ASR

IOTest IOTest

cGMP cGMP

PE

Clone: Thy1/310 (IgG1 Mouse)

Form

Format

Part#

Status

Line

Quality Standard

Size

PB

B30649 IM3703 B36121

0.5 mL

Liquid Liquid Liquid

ASR ASR ASR

IOTest IOTest IOTest

cGMP cGMP cGMP

PC5

1 mL

APC-A750

0.5 mL

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 79 Outside US Flow Cytometry Catalog