2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

CD85j, also called ILT2, is a member of the immunoglobulin (Ig)-like transcripts (ILT) family of genes located on human chromosome 19 (the numerical subclassification refers to the members in the order of the gene on the chromosome). It is a 110-kDa transmembrane glycoprotein. ILT2 receptor is a NK receptor involved in inhibition of NK and T cells cytolytic activity and cytokine production. ILT2 is also known as leucocyte Ig-like receptor (LIR)-1 and monocyte / macrophage Ig-like receptor (MIR) -7. The protein has 4 extracellular Ig-SF domains, and 4 cytoplasmic ITIM motifs in the cytoplasmic tail. Both myeloid and lymphoid cells express ILT2. Among lymphoid cells, NK cells, T cell subsets and all peripheral B cells express ILT2. It is also expressed on monocytes, macrophages, and dendritic cells. Ligands for CD85j are non-classical class I HLA-G molecules, certain alleles of HLA-A and -B loci and the human cytomegalovirus gene product, UL18, a viral homolog of HLA class I molecules. The inhibitory function of CD85j has been explored mainly in NK and T cells. In NK cells, ILT2 inhibits the cytolysis of HLA-Class I-positive target cells. In T cells, ILT2 inhibits both signaling and cellular events that control the activation of T cells. CD85j ( ILT2 )

Lysed normal whole blood sample.

Clone: HP-F1 (IgG1 Mouse)

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

A07408 B30645

1 mL

Liquid Liquid

ASR ASR

IOTest IOTest

cGMP cGMP

APC

0.5 mL

CD85k ( ILT3 )

The CD85k antigen (ILT3) is a member of Immunoglobulin (Ig)-Like Transcripts (ILT), also known as Leucocyte Ig-like Receptors (LIR) and Monocyte / Macrophage Ig-like Receptors (MIR). ILT are expressed on monocytes, macrophages, dendritic cells (DCs) and granulocytes, except ILT2, which is expressed by both myeloid and lymphoid cells: NK, T cell subsets and all peripheral B cells. All ILT receptors except (ILT6) are transmembrane proteins and have been classified in 3 types: Inhibitory: ILT2, 3, 4, 5, LIR8; Activating: ILT1, LIR6a, 7, LIR8, with a cytoplasmic tail associated to FcR γ ; Soluble: ILT6. The CD85k antigen (otherwise known as LIR-5, HM18) is a transmembrane protein of 60 kDa, constitutively phosphorylated. Unlike ILT2 and ILT4, ILT3 does not bind to HLA-A, -B and -G1 molecules. The ligand of ILT3 is unknown, however research studies point out the following functional properties: ILT3 is involved in antigen uptake and presentation; ILT3 negatively regulates Antigen Presenting Cell (APC) functional responses triggered via stimulatory receptors such as CD11b, CD16, MHC-class II. The transmission of a negative signal via ITIMs may be one of the inhibitory mechanisms, ranging from temporary to permanent inactivation, that control homeostasis, and immunological tolerance. It has been shown that ILT3, unregulated on monocytes and DCs by CD8+CD28- T suppressor cells, renders these APC tolerogenic.

Lysed normal whole blood sample.

Clone: ZM3.8 (IgG1 Mouse) The ZM3.8 antibody immunoprecipitates a protein of 55 kDa from ILT3-transfected T cells and a protein of 60 kDa from monocytes.

Form

Format

Part#

Status

Line

Quality Standard

Size

PC7

A46529

1 mL

Liquid

ASR

IOTest

cGMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 78 Outside US Flow Cytometry Catalog