2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

CD83

CD83, or HB15 molecule, is a 43 kDa glycoprotein member of the immunoglobulin superfamily. It is composed of a single extracellular V-type Ig-like domain, a transmembrane region and a 40 amino acid cytoplasmic domain. CD83 is predominantly expressed by dendritic lineage cells including Langerhans cells, skin and peripheral blood dendritic cells. It is also expressed by interdigitating reticulum cells present in the T cell zones of lymphoid organs as well as being weakly expressed by some germinal center lymphocytes. CD83 is expressed by in vitro generated dendritic cells. It is not expressed by resting peripheral blood leucocytes at detectable levels and is only expressed at low levels following lymphocyte activation in vitro.

K562 B10 Cell line.

Clone: HB15a (IgG2b Mouse) HB15a cross reacts with: Indian Rhesus, Common Marmoset, Squirrel Monkey.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC

IM2410U IM2218U IM3240U

2 mL 2 mL 1 mL

Liquid Liquid Liquid

ASR ASR ASR

IOTest IOTest IOTest

cGMP cGMP cGMP

PE

PC5

CD85d (ILT4) is a member of the new immunoglobulin (Ig)-like transcripts (ILT) family of genes located on human chromosome 19q13.4. ILT4 is also known as leucocyte Ig-like receptor (LIR)-2 and monocyte / macrophage Ig-like receptors (MIR)-10. ILT4 is a transmembrane protein recognized by 42D1 monoclonal antibody. The protein has extracellular Ig-SF domains, and ITIM motifs in the cytoplasmic tail that inhibit cellular responses by recruiting phosphatases such SHP-1. ILT4 is confined to the myelomonocytic lineage as it is expressed on monocytes, macrophages, and dendritic cells (DCs). ILT4 binds to HLA-G molecules with a 3- to 4-fold higher affinity than to classical MHC class I molecules. ILT4 may modulate one or several of the antigen presenting functions mediated by DCs, control inflammatory responses mediated by monocytes / macrophages or inhibit their cytotoxicity. The recognition of HLA-G by ILT4 may play a role in materno-fetal tolerance as HLA-G is selectively expressed in the trophoblast. It has been shown that recipient’s CD8+CD28– T suppressor (Ts) cells may induce tolerance by up-regulating ITL3 and ILT4 expressions in donor’s dendritic cells, rendering these antigen-presenting cells (APCs) tolerogenic. CD85d ( ILT4 )

Lysed normal whole blood sample.

Clone: 42D1 (IgG2a Rat)

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

A22334

100 tests

Liquid

RUO

IOTest

cGMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 77 Outside US Flow Cytometry Catalog