2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

CD80

The CD80 antigen (B7, BB1) is a highly glycosylated single-chain protein. Its extracellular domain consists of two Ig-like domains. This 60 kDa molecule shares with CD86 the capability to be the ligand for two structurally similar molecules expressed on T lymphocytes, CD28 and CD152 (CTLA-4). CD80 antigen is expressed on in vitro activated B lymphocytes after 24h stimulation and at maximal level 48-72h post activation. It is not expressed on the majority of resting B cells from peripheral blood but identifies a subpopulation of B cells that has been previously activated. The antigen is also expressed by HTLV-1 transformed T cells and activated monocytes. CD80 binding provides co-stimulatory signals for T cell activation.

RPMI8866 cell line.

Clone: MAB104 (IgG1 Mouse) TheMAB104monoclonalantibodyreactswithin vitroactivatedB lymphocytes,someB cell lines,andweaklywitha smallproportionof non-activatedB cells.This antibody also reacts with activated T cells but not with peripheral monocytes and T cells.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC

IM1853U IM1976U B30644 B30642 B30643

2 mL 2 mL

Liquid Liquid Liquid Liquid Liquid

ASR ASR ASR ASR ASR

IOTest IOTest IOTest IOTest IOTest

cGMP cGMP cGMP cGMP cGMP

PE

PC7 APC

0.5 mL 0.5 mL 0.5 mL

APC-A750

CD81

The CD81 molecule is a single chain, 4 transmembrane domain protein (Tetraspan/TM4SF). Both the NH2- and COOH-termini are located inside the cytoplasm, and two loops of the protein sequence are exposed extracellularly. The CD81 antigen is not glycosylated and its molecular weight is 26 kDa. Its tissue distribution is broad. This antigen may be present in some cases as multimolecular complexes, in association with other members of the TM4 superfamily (CD37, CD53) or, on the surface of B cells, in association with CD19 and/or CD21 and/or MHC class II antigens. Most B lymphocytes, at all stages of cellular differentiation, express CD81 at relatively high levels.

Lysed normal whole blood sample.

Clone: JS64 (IgG2a Mouse) The JS64 antibodyreacts with the majorityof normal lymphocytes,monocytesand eosinophils.Neutrophilsand plateletsare negative.JS64 has an anti- proliferative effect on some cell lines, but does not cause apoptosis. JS64 cross reacts with: Cynomolgus Monkey, Indian Rhesus, Olive Baboon.

Form

Format

Part#

Status

Line

Quality Standard

Size

PB

B19717 B25329 IM2579 A87789

0.5 mL

Liquid Liquid Liquid Liquid

ASR ASR ASR ASR

IOTest IOTest IOTest IOTest

cGMP cGMP cGMP cGMP

FITC

1 mL 2 mL

PE

APC

0.5 mL

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 76 Outside US Flow Cytometry Catalog