2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

The CD66c antigen, also known as non-specific cross-reacting antigen (NCA90), is a granulocyte cell surface GPI-linked protein of 80-100 kDa. It is also called KOR- SA3544. Along with CD66a, CD66b and CD66d, it is a member of the carcinoembryonic (CEA) antigen family which are involved in neutrophil activation and signaling and intercellular adhesion. It is also called CEACAM6. CD66c is a surface (and intracellular) molecule bound to the membrane by a glycosyl- phosphatidylinositol anchor. While its expression on peripheral granulocytes is well recognized, less is known about its distribution in early steps of normal and neoplastic hematopoiesis. It is not expressed by lymphocytes, monocytes, platelets or red blood cells. This antigen is never expressed by normal B cells. CD66c ( KOR-SA3544 )

Abnormal bone marrow sample. Graph kindly provided by The University of Tokyo Hospital.

Clone: KOR-SA3544 (IgG1 Mouse) In peripheralblood,the KOR-SA3544antibodyreactswith the majorityof granulocytesbut not with lymphocytes,monocytes,plateletsor red blood cells. In normal bone marrow, only the myeloid cells are positive.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC

IM2039U IM2357U

1 mL 1 mL

Liquid Liquid

ASR ASR

- -

cGMP cGMP

PE

CD69

The CD69 antigen is a 60 kDa disulfide-linked dimeric structure, known as activation inducer molecule (AIM). This structure contains two differentially glycosylated forms (33 and 27 kDa) of a single core protein, that are covalently associated. This activation antigen is one of the earliest appearing cell surface glycoproteins (before other activation antigens such as IL2-R) after T or B-lymphocyte activation but is absent in resting lymphocytes. It is also expressed by activated macrophages, NK cells and other cell types including neutrophils, eosinophils and platelets. Its expression in vitro can be induced by different factors such as PMA, PHA or CD3 monoclonal antibodies.

PHA cell line.

Clone: TP1.55.3 (IgG2b Mouse) TheCD69antibodyis usefulin combinationwithCD4,CD8andCD3antibodiesfor T cell activationstudies.TheTP1.55.3antibodyimmunoprecipitatesthe 60 kDa homodimer and both 27 kDa and 33 kDa subunits from activated peripheral blood lymphocytes under reduced and non-reduced conditions.

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

IM1943U 6607110 IM2656 A80710

2 mL

Liquid Liquid Liquid Liquid Liquid

ASR RUO

IOTest IOTest IOTest IOTest IOTest

cGMP cGMP

ECD PC5 PC7 APC

100 tests 100 tests

CE/IVD

98/79/EC

1 mL 1 mL

ASR ASR

cGMP cGMP

A80711

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 73 Outside US Flow Cytometry Catalog