2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

CD65

The CD65 antigen is analogous to the carbohydrate structure(s) recognized by CD15 antibodies. CD65 is a type II chain fucoganglioside involving the oligosaccharide sequence: Gal β 1-4GlcNac β 1-3Gal β 1-4GlcNac(Fuc α 1-3) β 1-R. CD65 has been suggested as the CD62L ligand, and it has been hypothesized to be involved in the extra vascular infiltration. CD65 is normally expressed on neutrophils, eosinophils, and basophils, on a monocyte subset and on a fraction of CD56bright NK cells. It is not expressed on lymphocytes. CD65 is also found on the membrane of adipocytes and on thymic epithelium but it is not expressed by mastocytes. In myeloid ontogenesis, CD65 seems to be expressed later than myeloperoxidase (MPO).

Lysed normal whole blood sample.

Clone: 88H7 (IgM Mouse) 88H7 antibody was re-assigned from the CD65s to the CD65 cluster during HLDA 6.

Form

Format

Part#

Status

Line

Quality Standard

Size

PB

B08164 B36299 IM1654U

0.5 mL

Liquid Liquid Liquid

ASR

IOTest IOTest IOTest

cGMP

FITC FITC

100 tests

CE/IVD

98/79/EC

2 mL

ASR

cGMP

CD66b

The CD66 antigens were originally described as granulocyte-specific activation antigens, related to the carcino-embryonic antigen (CEA) previously described on colon cancer cells. The CEA/CD66 gene group belongs to the immunoglobulin (Ig) gene superfamily. Among the CD66 cluster, CD66b (also known as CGM6) is a glycosyl-phosphatidylinositol (GPI)-anchored glycoprotein of 95-100 kDa. CD66b is thought to play a role in the regulation of the adhesive activity of CD11/CD18 via signal transduction in neutrophils. It is strongly expressed by myelocytes, metamyelocytes, neutrophils and polymorphonuclear neutrophils. CD66b is weakly expressed by promyelocytes.

Lysed normal whole blood sample.

Clone: 80H3 (IgG1 Mouse) The 80H3 antibody does not react with peripheral lymphocytes and monocytes.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC APC

IM0531U B15091 B08756

2 mL

Liquid Liquid Liquid

ASR ASR ASR

IOTest IOTest IOTest

cGMP cGMP cGMP

0.5 mL 0.5 mL

APC-A750

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 72 Outside US Flow Cytometry Catalog