2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

CD63

The CD63 antigen, also known as lysosomal membrane associated glycoprotein 3 (LAMP3), is a member of the tetraspanin (TM4SF) family. Nearly 20 genes encode tetraspanins whose main structural characteristics is their four transmembrane domains. CD63, as other tetraspanins (CD9, CD81, CD82), has recently been reported as forming complexes with VLA-3 and phosphatidylinositol 4-kinase, with VLA-6, CD11/CD18 and tyrosine kinase. CD63 was first described in granules of resting platelets and on the surface membrane on activated platelets. CD63 was later identified as granulophysin, a platelet dense granule glycoprotein. The molecular weight of the platelet form of CD63 ranges from 40 to 55 kDa. CD63 has been found to be identical to the ME491 antigen expressed by melanoma cells. It is also found on monocytes / macrophages and is weakly expressed by resting granulocytes, T lymphocytes and B lymphocytes. CD63 antigen, present in azurophilic granules of non-stimulated neutrophils, is strongly expressed on the surface of neutrophils after activation. Studies have shown that CD63 may be a marker for granule release. This phenomenom is also reported for basophils.

Lysed normal whole blood sample.

Clone: CLB-Gran/12 (IgG1 Mouse) The CD63 antibodies inhibit adhesion of neutrophils to activated endothelium. CLBGran/12 was used as a CD63 reference monoclonal antibody during HLDA 6.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC FITC

B92467 IM1165U IM1914U

100 tests

Liquid Liquid Liquid

CE/IVD

IOTest IOTest IOTest

98/79/EC

2 mL 2 mL

ASR ASR

cGMP cGMP

PE

CD64

CD64 is an integral membrane glycoprotein known as an Fc receptor that binds monomeric IgG-type antibodies with high affinity. It is also known as Fc-gamma receptor 1 (Fc γ RI) or FCRI. After binding IgG, CD64 interacts with an accessory chain known as the common γ chain, triggering cellular activation. Structurally, CD64 is composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type used to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 is constitutively found on macrophages and monocytes. CD64 is expressed on early myeloid cells and in IFN γ and G-CSF activated polymorphonuclear neutrophils (PMNs).

Lysed normal whole blood sample.

Clone: 22 (IgG1 Mouse) The22 antibodyshowsespeciallyhighaffinitybindingto humanmononuclearphagocytes.Thepitoperecognizedby thisantibodyis distinctfromtheIgGbinding site. 22 cross reacts with: Indian Rhesus.

Form

Format

Part#

Status

Line

Quality Standard

Size

PB

B19718 B49185 IM1604U IM3601U A98434 B49186 IM3606U B06025 B96769 A89308

0.5 mL

Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid

ASR

IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest

cGMP

FITC FITC

100 tests

CE/IVD

98/79/EC

2 mL 2 mL

ASR ASR ASR

cGMP cGMP cGMP

PE

ECD PC5 PC5 PC7

0.5 mL

100 tests

CE/IVD

98/79/EC

1 mL

ASR ASR

cGMP cGMP

0.5 mL 50 tests 0.5 mL

APC-A750 APC-A750

CE/IVD

98/79/EC

ASR

cGMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 71 Outside US Flow Cytometry Catalog