2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

CD62L

The CD62L antigen, also called LECAM-1, L-selectin or leucocyte adhesion molecule 1 [LAM-1], is a 75 kDa molecule, member of the selectin family. As other selectins (CD62E, CD62P), CD62L is a membrane-anchored Ca2+-dependent C-type lectin that binds to cell-surface carbohydrate ligands. CD62L acts as the peripheral lymph node homing receptor. It is also involved in lymphocyte binding to High Endothelial Venules (HEV) and in lymphocyte rolling on activated endothelium. CD62L is expressed by B and T lymphocytes, monocytes, neutrophils, eosinophils, and approximately half of the peripheral NK lymphocytes. It is also expressed by some spleen and bone marrow lymphocytes, as well as by some thymocytes and bone marrow myeloid cells. The expression level of CD62L on lymphocytes may be subject to control mechanisms such as downregulation and/or upregulation. On neutrophils, monocytes and their bone marrow precursors, CD62L is also downregulated by stimulation with granulocyte-macrophage colony stimulating factor (GM-CSF).

Lysed normal whole blood sample. Gated on lymphocytes.

Clone: DREG56 (IgG1 Mouse) In contrastto otherantibodiesrecognizingthis molecule,DREG56is particularlyeffectivein blockingCD62Lmediatedlymphocytebindingto lymphnodeHEV. DREG56 (ref.33) was used as a CD62L reference monoclonal antibody during the HLDA 6. DREG56 cross reacts with: Chimpanzee and Bovine.

Form

Format

Part#

Status

Line

Quality Standard

Size

PB

B30640 IM1231U IM2214U IM2713U IM2655U B30641 B30639 B26604

0.5 mL

Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid

ASR ASR ASR ASR ASR ASR ASR ASR

IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest

cGMP cGMP cGMP cGMP cGMP cGMP cGMP cGMP

FITC

2 mL 2 mL 1 mL 1 mL

PE

ECD PC5 PC7 APC

0.5 mL 0.5 mL 0.5 mL

APC-A750

CD62P

The CD62P antigen (P-selectin/GMP-140/PADGEM) is a member of the selectin family. Like other selectins (CD62E and CD62L), CD62P contains an amino terminal lectin-like domain, followed by an EGF domain, nine short consensus repeats (SCR), a transmembrane domain and a short cytoplasmic domain. CD62P (140 kDa) is present in megakaryocytes, in Weibel-Palade bodies of endothelial cells and in α -granules of platelets. CD62P is translocated to the surface membrane upon in vitro and in vivo activation. CD62P is involved in the interaction between activated platelets and with monocytes and neutrophils.

Platelet rich plasma (PRP) from a normal whole blood sample.

Clone: CLB-Thromb/6 (IgG1 Mouse) TheCLB-Thromb/6antibodyrecognizestheboundaryregionbetweenthelectinandEGF-likedomains,whichis preservedafterplateletfixation.It inhibitsplatelet adhesion to eosinophils in cell adhesion assays.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC

A07790 IM1759U

100 tests

Liquid Liquid

CE/IVD

IOTest IOTest

98/79/EC

PE

2 mL

ASR

cGMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 70 Outside US Flow Cytometry Catalog