2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

CD49b

The CD49b antigen is a transmembrane glycoprotein of 150 kDa also known as the α -2 integrin and as platelet GPIa. CD49b non-covalently pairs with the β -1 integrin (CD29, GPIIa) to form the VLA-2 (very late activation antigen) complex. CD49b, was first identified as an extracellular matrix receptor for collagens and/or laminins. It is now recognized that the α 2 β 1 integrin serves as a receptor for many matrix and nonmatrix molecules. Extensive anlayses have defined distinct conformations that lead to inactive, partially active or highly active ligand binding. The mechanisms by which the α 2 β 1 integrin plays a critical role in platelet function and homeostasis have been carefully defined. Studies have confirmed human physiology and disease states mediated by this receptor in immunity, cancer, and development. CD49b is expressed by platelets, activated T lymphocytes and thymocytes.

A431 cell line.

Clone: Gi9 (IgG1 Mouse) TheGi9antibodyreactswiththemoleculecarryingthe Br(a)as wellas the Br(b)alloantigen.Thisantibodyinhibitsthe adhesionof plateletsto collagen.It detects humanalloantibodies(anti-Bra,b) againstVLA-2by MAIPAassay.Gi9 was usedas a CD49breferencemonoclonalantibodyduringthe HLDA6. Gi9 reactswith platelets and weakly with monocytes. It also stains most adherent cell lines and T lymphocytes in culture.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC

IM1425 IM0717

100 tests

Liquid

RUO RUO

IOTest

cGMP cGMP

UNLB

0.2 mg

Freeze-dried

-

CD49d

CD49d is the integrin α 4 chain that non-covalently pairs with CD29 ( β 1 chain) to form the very late activation antigen 4 (VLA-4), or with the integrin β 7 chain. CD49d is a transmembrane glycoprotein of 145 kDa devoid of disulfide bond. Complexed to β 1 or β 7 integrins, CD49d is not only involved in cell adhesion to fibronectin and vascular cell adhesion molecule 1 (VCAM-1), but also in intercellular leucocyte interactions. It is expressed by monocytes, lymphocytes, eosinophils, thymocytes and Langerhans cells.

Lysed normal whole blood sample.

Clone: HP2/1 (IgG1 Mouse) The HP2/1antibodyblocksthe interactionof VLA-4with VCAM-1.HP2/1was used as a CD49dreferencemonoclonalantibodyduringthe HLDA6. HP2/1cross reacts with: Indian Rhesus and Bovine, Ovine, Caprine, Equine, Canine, Feline, Mink, Llama, Rabbit, Carp.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC APC

IM1404U B01682

2 mL

Liquid Liquid Liquid

ASR ASR ASR

IOTest IOTest IOTest

cGMP cGMP cGMP

0.5 mL 0.5 mL

APC-A750

B16893

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 65 Outside US Flow Cytometry Catalog