2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

CD43

The CD43 antigen is a 95-135 kDa heavily O-glycosylated and sialylated single chain membrane glycoprotein. This cell surface mucin-type glycoprotein is also called leukosialin or sialophorin. CD43 antigen is expressed by T lymphocytes, plasma cells, neutrophils, weakly on resting B lymphocytes but highly after activation, and on platelets. A soluble form of CD43 (galactoglycoprotein) can be detected in plasma. This form is produced by proteolytic shedding of the extracellular portion of the membrane molecule after cell activation. Studies, coupled with recent evidence that CD43 influences cellular adhesion, indicate that differential expression of CD43 may play a role in monocyte/macrophage trafficking.

Lysed normal whole blood sample.

Clone: DFT1 (IgG1 Mouse) TheDFT1antibodyreactswithan extracellularepitopeof the CD43antigen.It is usefulfor detectingleucocytesby flowcytometryandimmunohistochemistryon routinely-fixedand parrafin-embeddedtissue sections.The DFT1 antibodyis also able to induce homotypicadhesionof leucocytes.DFT1 cross reacts with: Cynomolgus Monkey, Indian Rhesus, Olive Baboon and Ovine.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC

IM3264U A32560 B49195 A89307

2 mL 2 mL

Liquid Liquid Liquid Liquid

ASR ASR

IOTest IOTest IOTest IOTest

cGMP cGMP

PE

APC-A750 APC-A750

50 tests 0.5 mL

CE/IVD

98/79/EC

ASR

cGMP

CD44

The CD44 is a transmembrane glycoprotein of 85-200 KDa. Multiple CD44 isoforms have been described. CD44 is present on most cells or tissues, but not on platelets, hepatocytes, cardiac muscle, kidney tubular epithelium, testis and skin portions. CD44 is a signaling platform that integrates cellular microenvironmental cues with growth factor and cytokine signals. It transduces signals to membrane-associated cytoskeletal proteins or to the nucleus to regulate a variety of gene expression levels related to cell-matrix adhesion, cell migration, proliferation, differentiation, and survival. This cell adhesion molecule plays an important role in tumor progression and metastasis. Its role in tumorigenesis is due to its binding to extracellular matrix components, including hyaluronan (HA) and osteopontin (OPN), and to messenger molecules, such as growth factors present in the tumor microenvironment. The human Indian (In) blood group antigens a/b reside on CD44.

Lysed normal whole blood sample.

Clone: J.173 (IgG1 Mouse) J.173antibodydoesnotinhibitthebindingof hyaluronateto its receptor.It cantriggerIL-2-dependentproliferationandcytotoxicityof humanT cellclones

in vitro .

J.173 cross reacts with: Cynomolgus Monkey, Indian Rhesus, Olive Baboon.

Form

Format

Part#

Status

Line

Quality Standard

Size

PB

B37789 IM1219U A32537 B30637

0.5 mL

Liquid Liquid Liquid Liquid

ASR ASR ASR ASR

IOTest IOTest IOTest IOTest

cGMP cGMP cGMP cGMP

FITC

2 mL 2 mL

PE

APC-A750

0.5 mL

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 61 Outside US Flow Cytometry Catalog