2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

CD42a

The CD42a antigen (GPIX) is a single-chain transmembrane glycoprotein of 22 kDa that forms a noncovalent complex with CD42b, CD42c and CD42d (GPIb α , GPIb β , and GPV). The CD42a expression is restricted to platelets and megakaryocytes. The GPIb-IX-V complex is the platelet receptor for von Willebrand factor and is known as von Willebrand's factor-dependent adhesion receptor. Although GPIb-IX-V primarily functions as a platelet receptor for von Willebrand factor (VWF), it can also bind to other ligands present in circulation such as thrombin, P-selectin, integrin α M β 2, factor XI, factor XII, high- molecular-weight kininogen, as well as a number of snake venom proteins.

Platelet rich plasma from a normal whole blood sample.

Clone: SZ1 (IgG2a Mouse) The SZ1 monoclonal antibody reacts with the CD42a-d complex but does not recognize GPIb or GPIX individually.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC

IM1757U IM0538

2 mL

Liquid

ASR RUO

IOTest

cGMP cGMP

UNLB

0.2 mg

Freeze-dried

-

CD42b

The CD42b antigen (GPIb α ) is a membrane glycoprotein of 135 kDa, that forms a disulfide linked 160 kDa membrane protein with the CD42c (GP1b β ) known as GP1b. GP1b forms a noncovalent complex with GPIX (CD42a) and GPV (CD42d). This antigen is found on platelets and megakaryocytes. It has also been reported on vascular and tonsillar endothelial cells. The CD42 complex serves as the von Willebrand Factor(vWF) surface receptor involved in the adhesion of platelets to the subendothelium of damaged vascular walls.

Platelet rich plasma (PRP) from a normal whole blood sample.

Clone: SZ2 (IgG1 Mouse) The SZ2 monoclonalantibody prevents the ristocetin-dependentbinding of von Willebrand'sfactor to platelets and inhibits ristocetin-inducedplatelet agglutination.Plateletaggregationinducedby type I collagenand PlateletActivatingFactor(PAF)is also inhibited.The SZ2 antibodyimmunoprecipitatesthe components of the GPIb complex. It may also be used in Western blot.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC

IM0648U IM1417U B13980 IM0409

2 mL 2 mL

Liquid Liquid Liquid

ASR ASR ASR RUO

IOTest IOTest IOTest

cGMP cGMP cGMP cGMP

PE

APC

0.5 mL 0.2 mg

UNLB

Freeze-dried

-

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 60 Outside US Flow Cytometry Catalog