2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

CD27

The CD27 molecule is a transmembrane disulfide-linked homodimer which belongs to tumor necrosis factor receptor (TNFR) / nerve growth factor receptor (NGFR) family. The molecular weight of the recognized antigen is 55 kDa / 120 kDa under reducing / non reducing conditions respectively. The CD27 glycoprotein is found on medullary thymocytes, peripheral T cells, subset of mature B cells and NK cells. On T cells, CD27 is preferentially expressed on the CD45RA+ CD45RO- naive subset of CD4+ T lymphocytes whereas most memory T cells (CD45RA- CD45RO+) lack CD27. Activation of T cells, when involving CD27 molecule, results in the upregulation of CD27 expression as cell surface embedded, and also in the release of a soluble form of CD27 (sCD27). CD70 antigen, which is a member of the TNF ligand superfamily, interacts with CD27 molecule and is known to be the CD27-ligand.

Lysed normal whole blood sample.

Clone: 1A4CD27 (IgG1 Mouse) 1A4CD27 cross reacts with: Cynomolgus Monkey, Indian Rhesus, Olive Baboon.

Form

Format

Part#

Status

Line

Quality Standard

Size

PB PE PE

B30635 B96790 IM2578 B26603 6607107 B21444 B49205 A54823 B09983

0.5 mL

Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid

ASR

IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest

cGMP

100 tests

CE/IVD

98/79/EC

2 mL

ASR ASR RUO ASR ASR ASR ASR

cGMP cGMP cGMP cGMP cGMP cGMP cGMP

ECD PC5

0.5 mL

100 tests

PC5.5

0.5 mL

PC7 PC7 APC

100 tests

CE/IVD

98/79/EC

1 mL

0.5 mL 0.5 mL

APC-A750

B12701

CD28

The CD28 antigen (Tp44) is a homodimer of disulfide-linked chains, with a molecular weight of 44 kDa each. It is involved in the interaction of T lymphocytes with antigen- presenting cells (APCs), through its counter-receptors, B7-1/BB-1 (CD80) and B7 -2/B70 (CD86). It provides a major co-stimulatory signal for T cell activation, proliferation and lymphokine production. The CD28 family of receptors (CD28, CTLA -4, ICOS, PD-1 and BTLA) plays a critical role in controlling the adaptive immune response. The CD28 receptor can enhance T cell antigen receptor (TCR) signals, as well as deliver independent signals. Although the signals through CD28 are crucial for the initial co-stimulation of interleukin-2 (IL-2) production, a TCR-independent CD28 signal leads to the selective transcription of survival, but not proliferative genes. CD28 is expressed by a majority of CD3+ T lymphocytes. CD4+ T lymphocytes express the CD28 antigen more frequently than do CD8+ T lymphocytes. CD28 antigen is also present on plasma cells and thymocytes.

Lysed normal whole blood sample.

Clone: CD28.2 (IgG1 Mouse) The CD28.2monoclonalantibodycostimulatesT cell proliferationand cytokineproductionin the presenceof T cell activators( α proliferation of CD4 + T lymphocytes. It cross-reacts with Rhesus monkey peripheral blood lymphocytes.

CD3, PMA, SEB). It inhibits

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC

IM1236U IM2071U 6607111 6607108 B24027 B23313 B10244 B12696 B08757 IM1376

2 mL 2 mL

Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid

ASR ASR RUO RUO ASR ASR ASR ASR ASR ASR

IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest

cGMP cGMP cGMP cGMP cGMP cGMP cGMP cGMP cGMP cGMP

PE

ECD PC5

100 tests 100 tests

PC5.5

0.5 mL 0.5 mL 0.5 mL 0.5 mL 0.5 mL 0.2 mg

PC7 APC

APC-A700 APC-A750

UNLB

Freeze-dried

-

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 51 Outside US Flow Cytometry Catalog