2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

CD16b

Fc- γ RIII (CD16) is a low affinity receptor involved in interactions with immune complexes. It is a 50 – 80 kDa protein with two distinct forms encoded by two different genes: IIIA and IIIB. The transmembrane form (Fc- γ RIIIA) is expressed on NK cells, monocytes and macrophages. The IIIB mRNA encodes Fc γ RIIIB, a glycoprotein anchored to the outer leaflet of the membrane by a glycosylphosphatidyl-inositol (GPI) moiety and is transcribed only in human neutrophils. Fc γ RIIIB (CD16b) may act with Fc γ RIIA (CD32) expressed on neutrophils to mediate antibody-dependent cytotoxicity (ADCC) and phagocytocis. The GPI-linked receptor exists in a soluble form (sCD16) revealing an immunoregulatory function through interaction with complement receptors.

Lysed normal whole blood sample.

Clone: 1D3 (IgM Mouse) Themonoclonalantibody1D3wasshownto be non-reactivewithNK cellsor monocytesbutto be specificto polymorphonuclearleucocytes(PMNs).It recognizes an epitopespecificto theFc γ RIIIBmoleculeandwascharacterizedas thesoleantibodybelongingto theCD16bclusterof differentiation.Thisantibodyreactswith PMNs,irrespectiveof the neutrophilantigen(NA)phenotype,althoughit showslowerreactivitywithNA2-homozygotePMNsas comparedto NA1-homozygotes or NA1/NA2 heterozygotes.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC

IM2353U

2 mL

Liquid

ASR

IOTest

cGMP

CD18

The beta2 integrins (CD11/CD18) are the major adhesion molecule family of leukocytes. Most leukocytes express one or more members of this family. CD18 is the 95 kDa integrin β 2-chain which forms non-covalently-bound heterodimers with one of four α subunits. The four alpha subunits are: CD11a ( α L) (all leukocytes), CD11b ( α M) (granulocytes, monocytes, some macrophages), CD11c ( α X) (granulocytes, monocytes, dendritic antigen presenting cells), CD11d ( α D) (CD8 T-cell subpopulation including large granular lymphocytes, macrophages and gamma-delta T cells in splenic red pulp). CD18 is expressed by all leukocytes (including histiocytes - macrophages, Langerhan's cells and dendritic cells) but no platelets. Macrophages and granulocytes express 10-fold more CD18 than do lymphocytes. Clone: 7E4 (IgG1 Mouse) 7E4 is a blockingmonoclonalantibodyin cell adhesionassays.7E4 crossreactswith:CynomolgusMonkey,IndianRhesus,HamadyrasBaboon,OliveBaboonand Bovine, Ovine, Porcine, Equine, Canine, Feline, Mink, Llama.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC

IM1568U IM1570U

2 mL 2 mL

Liquid Liquid

ASR ASR

IOTest IOTest

cGMP cGMP

PE

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 45 Outside US Flow Cytometry Catalog