2020 Outside US Flow Cytometry Catalog

DURAClone Panels

Size

Status

Part #

DURAClone IM Treg Tube

B53346

25 tests

RUO

Regulatory T cells (Tregs) play a crucial role in the induction and maintenance of immunological tolerance. Together with CD4 and CD25, the expression of the transcription factor FoxP3 is considered the hallmark of human regulatory T cells (CD3+ CD4+ CD25+ FoxP3+). Helios, a transcription factor of the Ikaros family, has been described as a marker identifying thymus-derived nTregs - as opposed to Tregs induced in peripheral tissues (iTregs). Additionally, Helios acts as an enhancer of regulatory function. Surface expression of ectoenzyme CD39 exerts a suppressive function by its ATPase activity and is restricted to an effector/memory-like subpopulation of Tregs. CD45RA surface expression indicates a naïve or unprimed subpopulation of Tregs. The DURAClone IM Treg Tube is an 8-color, 8-monoclonal antibody reagent that allows the identification of subpopulations of regulatory T cells in human whole blood samples by eight color flow cytometry. It contains 3 Compensation Kits, each kit containing eight tubes, each of a single color: CD4-FITC; CD4-PE; CD39-PC5.5; CD4-PC7; FoxP3-A647; CD3-APC-A750; CD4-Pacific Blue; CD8-Krome Orange. All tandem dye-labeled antibodies are lot-matched.

Fluorochrome

Clone (Isotype)

Specificity

CD45RA

FITC

2H4LDH11LDB9 (IgG1 Mouse)

CD25

PE

B1.49.9 (IgG2a Mouse)

CD39

PC5.5

BA54 (IgG1 Mouse)

CD4

PC7

SFCI12T4D11 (IgG1 Mouse)

FoxP3

Alexa Fluor* 647

259D (IgG1 Mouse)

CD3

APC-Alexa Fluor* 750

UCHT1 (IgG1 Mouse)

Helios

Pacific Blue*

22F6 (IgG Hamster)

CD45

Krome Orange

J33 (IgG1 Mouse)

Not available in all geographies. DURAClone Panels are available for Research Use Only. Not for use in diagnostics procedures.

ANTIBODIES & KITS

170

Outside US Flow Cytometry Catalog