2020 Outside US Flow Cytometry Catalog

DURAClone Panels

Size

Status

Part #

DURAClone IM TCRs Tube

B53340

25 tests

RUO

Mature T cells - as identified by positive surface CD3 staining - express T cell receptors (TCRs) that enable low affinity-recognition of antigens bound to major histocompatibility complex (MHC) molecules. MHC co-binding of either CD4 (MHC class II) or CD8 (MHC class I) enhances and prolongs the engagement with the antigen-presenting cells. Two forms of TCRs are known either consisting of heterodimers of variable α and β protein chains or γ and δ protein chains. Proportions of 95% αβ T cells and 5% γδ T cells are commonly found in blood .The largest subset of γδ T cells can be identified by the IMMU 389 antibody clone recognizing the γδ complex from the V δ 2+ T cell receptor (TCR γδ V δ 2+), being mostly associated with V γ 9. A smaller subset of γδ T cells is staining positive with the R9.12 antibody clone that binds to the V δ 1 segment of γδ T cells (TCR γδ V δ 1+). The relative sizes of these two subsets have been assigned a role in feto-maternal and allograft tolerance. The DURAClone IM TCRs Tube is a 9-color, 9-monoclonal antibody reagent that allows the identification of the TCR subtypes on the surface of mature T cells. It contains 3 Compensation Kits, each kit containing 9 tubes, each of a single color: CD4-FITC, CD4-PE, HLA DR-ECD, V δ 1-PC7, CD4-APC, CD8-A700, CD3-APC-A750, CD4-Pacific Blue, CD8-Krome Orange. All tandem dye-labeled antibodies are lot-matched.

Fluorochrome

Clone (Isotype)

Specificity

TCR γδ

FITC

IMMU510 (IgG1 Mouse)

TCR αβ

PE

IP26A (IgG1 Mouse)

HLA-DR

ECD

Immu-357 (IgG1 Mouse)

TCRV δ 1

PC7

R9.12 (IgG1 Mouse)

CD4

APC

13B8.2 (IgG1 Mouse)

CD8

Alexa Fluor* 700

B9.11 (IgG1 Mouse)

CD3

APC-Alexa Fluor* 750

UCHT1 (IgG1 Mouse)

TCRV δ 2

Pacific Blue*

IMMU 389 (IgG1 Mouse)

CD45

Krome Orange

J33 (IgG1 Mouse)

Not available in all geographies. DURAClone Panels are available for Research Use Only. Not for use in diagnostics procedures.

ANTIBODIES & KITS

169

Outside US Flow Cytometry Catalog