2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

Thrombin receptor

Thrombin is a coagulation protease produced at sites of vascular injury that activates platelets, endothelial cells, leukocytes and mesenchymal cells via cleavage of specific cell surface receptors known as proteinase activated receptors (PARs). A functional thrombin receptor from human platelets has been cloned and sequenced. It is a 66- kDa, single polypeptide chain that belongs to the cell surface G-protein-coupled receptor family, with seven transmembrane domains and an extracellular N terminus. The thrombin cleavage site is located in the N terminus between Arg41 and Ser42. Following cleavage by thrombin, activated receptors undergo desensitization and internalization but a fraction of them are recycled to the cell surface. The Thrombin Receptor is expressed on a variety of cells including platelets and endothelium. The receptor is a substrate of thrombin and has been shown to play a role in the activation of platelets.

HEL Cell line.

Clone: SPAN 12 (IgG1 Mouse) TheSPAN12 antibodyrecognizesan epitopeon the receptorthatspansthe thrombincleavagesite.It bindsto intactreceptors,but not to cleavedones.SPAN12 inhibits thrombin-induced receptor activation at reduced thrombin concentrations.

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

IM2583

2 mL

Liquid

ASR

IOTest

cGMP

Clone: WEDE15 (IgG1 Mouse) TheWEDE15antibodyrecognizesan epitopeon the receptor,distantfromthe N-terminalthrombincleavagesite (WEDEE).It reactswithbothintactandcleaved receptors.

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

IM2584

2 mL

Liquid

ASR

IOTest

cGMP

TIA-1

The T cell intracellular antigen 1 (TIA-1) is a 17 kDa cytoplasmic granule-associated protein, expressed in cells possessing cytolytic potential. TIA-1 is also designated as GMP-17 (granule membrane protein of 17 kDa). The GMP-17/TIA-1 molecule could be involved in the molecular signaling cascade during Fas (CD95)-mediated apoptosis. In normal peripheral blood lymphocytes, TIA-1 is expressed by 90% of CD16+, 50-60% of CD8+, and less than 10% of CD4+ cells. Although TIA-1 may be detected in unstimulated T lymphocytes, activated T lymphocytes are induced to express disulfide linked polymers of the 17 kDa protein, also recognized by TIA-1. TIA-1 is expressed by all monocytes and granulocytes, by activated CD4+ T-cell clones and activated natural killer cell clones, and by Con A-activated thymocytes. On the contrary, TIA-1 is not expressed by B lymphocytes or B-cell lines.

Lysed normal whole blood sample.

Clone: 2G9A10F5 (IgG1 Mouse) The 2G9 antibodyis a useful reagentfor detectingcytolyticeffectorcells in tissue infiltrates.It was evaluatedduring HLDA 5 in the sectionof monoclonal antibodiesreactive with intracellularantigens. It may be used to detect intracytoplasmicTIA-1 in flow cytometryafter permeabilizationwith IntraPrep permeabilization reagent.

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

IM3293 IM2550

2 mL

Liquid

ASR ASR

IOTest

cGMP cGMP

UNLB

0.2 mg

Freeze-dried

-

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 142 Outside US Flow Cytometry Catalog