2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

Lactoferrin

Lactoferrin is a 76 kDa single chain polypeptide with iron-binding property. This protein shows structural similarity to the plasma iron-transport protein transferrin. Lactoferrin is involved in various immuno-regulatory functions associated with anti- infective and inflammatory responses such as antibody synthesis, cytokine production, NK cell cytotoxicity, complement activation and lymphocyte proliferation. Lactoferrin synthesis occurs at the myelocytic stage during polymorphonuclear neutrophil (PMN) ontogeny and constitutes a component of secondary (or specific) granules. Unlike the myeloperoxydase, lactoferrin is absent from primary (or azurophilic) granules and appears later during maturation of the granulocytic lineage. Thus, in peripheral blood, intracellular lactoferrin expression is restricted to neutrophils.

Lysed normal whole blood sample.

Clone: CLB13.17 (IgG1 Mouse) ThePE-conjugatedCLB-13.17antibodyis optimizedfor flowcytometricdetectionof intracellularlactoferrinby flowcytometryafterpermeabilizationwithIntraPrep permeabilization reagent.

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

IM2742

2 mL

Liquid

ASR

IOTest

cGMP

Myeloperoxidase

Myeloperoxidase (MPO) is an heterodimeric glycoprotein of 150 kDa with an α 2/ β 2 structure. The two subunits ( α and β ) have a molecular weight of 55 and 15 kDa, respectively. MPO synthesis occurs in bone marrow at an early stage of myeloid lineage differentiation. MPO is specifically expressed during promyelocytic formation, the stage at which azurophilic granules (or primary granules) are formed. MPO is still found in mature myeloid cells, becoming the major constituent of azurophilic granules of neutrophils. MPO is stored in polymorphonuclear neutrophilic granules and in macrophages, but it is not expressed in lymphocytes, platelets and erythrocytes. MPO catalyzes the formation of hypochlorous acid (HOCl) in the presence of active oxygen (H2O2). Intranuclear MPO may also help to protect DNA against damage resulting from oxygen radicals produced during cell maturation and function.

Permeabilized normal whole blood sample.

Clone: CLB-MPO-1 (IgG2a Mouse) The CLB-MPO-1antibodyrecognizesMPO and its enzymaticallyinactiveform. This antibodycan be used to detectintracytoplasmicmyeloperoxidasein flow cytometry after permeabilization with IntraPrep or PerFix-nc permeabilization reagent.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC FITC

IM1874

100 tests

Liquid Liquid Liquid Liquid Liquid

CE/IVD

IOTest IOTest IOTest IOTest IOTest

98/79/EC

IM1874U B36288 IM3455U

2 mL

ASR

cGMP

PE PE

100 tests

CE/IVD

98/79/EC

2 mL

ASR ASR

cGMP cGMP

PC5.5

B23132

0.5 mL

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 122 Outside US Flow Cytometry Catalog