2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

Ki-67

Ki-67 nuclear antigen is associated with cell proliferation and is found throughout the cell cycle (G1, S, G2, M phases). It is never expressed by resting (G0) cells. The Ki-67 antigen is a human nuclear protein defined by its reactivity with monoclonal antibody from the Ki-67 clone. Two isoforms of 345 and 395 kDa have been identified by cDNA sequence coding. Deduced amino acid sequence analysis of the Ki-67 antigen confirmed that the cDNA encodes for a nuclear and short-lived protein without any significant homology to known sequences. The Ki-67 antigen is expressed at active phases of the cell cycle (G1, S, G2 and M phases), but it is absent in resting cells (G0 phase). The level of Ki-67 antigen expression varies during cell cycle and it has been correlated to several cell pathways: The Ki-67 decrease pathway is characterized by a declining Ki-67 staining and leads eventually to the exit from the active cell cycle (G0). If cells on this pathway get stimulated by growth factors, they can enter the Ki -67 increase pathway that brings the cells back into S phase. Cells following the Ki-67 stable pathway exhibit a constant intensity of Ki-67 staining during the G1 phase. This pathway is thought to correspond to optimal local growth conditions. The cellular localization of the Ki-67 protein is cell cycle phase dependent. During interphase, the antigen can be exclusively detected within the nucleus, whereas in M phase most of the protein is relocated to the surface of the chromosomes. The antigen is rapidly degraded as the cell enters the non proliferative state.

Lysed normal whole blood sample spiked with MOLT4 cell line.

Clone: Ki-67 (IgG1 Mouse)

Form

Format

Part#

Status

Line

Quality Standard

Size

AF488

B68180

0.5 mL

Liquid

ASR

IOTest

cGMP

The Lambda light chain of immunoglobulins is expressed at the surface of a cellular sub-population corresponding to approximately 1/3 of mature B lymphocytes in peripheral blood. This light chain is also found on the surface of a sub-population of immature bone marrow B lymphocytes. λ Chain

DAUDI / NAMALWA Cell line.

Clone: Polyclonal (F(ab’) 2

Goat)

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

C15189 B90416

2 mL

Liquid Liquid

ASR ASR

IOTest IOTest

cGMP cGMP

PC7

0.5 mL

Clone: Polyclonal (F(ab’) 2

Rabbit)

Form

Format

Part#

Status

Line

Quality Standard

Size

PE PE

B49174 A64827

100 tests

Liquid Liquid

CE/IVD

IOTest IOTest

98/79/EC

2 mL

ASR

cGMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 121 Outside US Flow Cytometry Catalog