2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

The anti-IgE mAb binds to circulating IgE as well as to IgE antibodies bound to the low affinity receptor Fc ε RII (expressed mainly on eosinophils, B lymphocytes), or to the high affinity receptor Fc ε RI (expressed on mast cells, basophils, monocytes and monocytes-derived cells). The anti-IgE antibody induces the histamine release from basophils. However, it is known in the literature information that basophils from 10% to 20% of donors completely fail to release histamine in response to anti-IgE, designated anti-IgE "non releasers". IgE (D ε 2)

Lysed normal whole blood sample.

Form Clone: E124.2.8 (IgG1 Mouse) The E124.2.8 monoclonal antibody binds specifically to the D ε 2 constant domain of human Immunoglobulin E (IgE) (D ε 2 is the heat labile region of IgE). Part# Format Size Status Line Quality Standard UNLB - ASR A40174 Freeze-dried 0.2 mg cGMP

IgM

The anti-IgM antibody binds to circulating IgM as well as to IgM antibodies bound to the Fc μ R. The Fc μ R is a transmembrane single sialoglycoprotein of 60 kD with O- linked oligosaccharides. It contains an extracellular Ig-like domain homologous to two other IgM-binding receptors (polymeric Ig receptor and Fc α / μ R) but exhibits an exclusive Fc μ -binding specificity. Unlike other FcRs, the major cell types expressing Fc μ R are adaptive immune cells, including B and T lymphocytes. The Fc μ R can be expressed as a cell surface activation antigen throughout the pre-B and B cell stages in differentiation. Receptor expression is not directly linked with IgM production, as both μ - pre-B cells and isotype-switched B cells may express the Fc μ R. The Fc μ R is thus the third member of a family of Fc receptors expressed on B-lineage cells, and its preferential expression on activated B cells suggests a potential role in the response to antigens. Clone: SA-DA4 (IgG1 Mouse) The SA-DA4 monoclonal antibody binds specifically to mu heavy chain of Human Immunoglobulin (IgM).

Pre-washed lysed normal whole blood sample.

Form

Format

Part#

Status

Line

Quality Standard

Size

PB

B30656 B30655 B30657 B30654

50 tests 50 tests 50 tests 50 tests

Liquid Liquid Liquid Liquid

RUO RUO RUO RUO

IOTest IOTest IOTest IOTest

cGMP cGMP cGMP cGMP

FITC

PE

APC

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If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 118 Outside US Flow Cytometry Catalog