2020 Outside US Flow Cytometry Catalog

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Single-Color Conjugated Antibodies

HLA-ABC

HLA-A, -B, and -C are major histocompatibility complex (MHC)-class I antigens. Like other class I molecules (i.e. HLA-E, -F, -G), HLA-A, -B, and -C are heterodimers consisting of a 40 – 45 kDa transmembrane glycoproteic α -chain, noncovalently combined to the invariant β 2-microglobulin. All class I molecules have conserved, monomorphic domains, but are also characterized by their extensive degree of allelic polymorphism. MHC molecules play a central role in the immune response. They are involved in the maturation of T cell repertoire, in the activation of T lymphocytes by presentation of xenogenic peptides or in the allogenic response. HLA-A, -B and -C are “classical” MHC Class I molecules and are expressed on the surface of most nucleated human cell types.

Lysed normal whole blood sample.

Clone: B9.12.1 (IgG2a Mouse) The B9.12.1monoclonalantibodyrecognizesa monomorphicepitopecommonto HLA-A,-B and -C molecules.B9.12.1antibodydoesnot recognizeunassociated heavy chains. B9.12.1 cross reacts with: Cynomolgus Monkey, Indian Rhesus, Olive Baboon.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC

IM1838U IM0107

2 mL

Liquid

ASR RUO

IOTest

cGMP cGMP

UNLB

0.2 mg

Freeze-dried

HLA-DQ

The HLA (Human Leucocyte Antigen) system is the name given to the major histocompatibility complex (MHC) in man. The MHC complex is located on the short arm of the chromosome 6 and encodes three groups of molecules designated MHC class I, class II and class III. Coded by 5 loci (DM, DO, DP, DQ and DR) of the D locus, HLA class II molecules are also called HLA-DM, HLA-DO, HLA-DP, HLA-DQ and HLA- DR antigens. The HLA class II molecules like class I molecules, are composed of non- covalently associated α / β heteterodimers. On HLA class II, both heavy ( α ) and light ( β ) chain with a molecular weight of 31-33 kDa and 26-29 kDa respectively span the cell membrane. The two immunoglobulin-like domains proximal to the cell membrane ( α 2 and β 2) support the two polymorphic amino-terminal domains ( α 1 and β 1) distal to the membrane which constitute a part of the "antigen presenting site". HLA class II molecules are involved in the regulation of immune response whose expression is limited to antigen presenting cells, i.e. B lymphocytes, monocytes / macrophages, dendritic and Langerhans cells of the skin.

Lysed normal whole blood sample.

Clone: SPVL3 (IgG2a Mouse) The monoclonalantibodySPVL3recognizesa monomorphicdeterminantof humanclassII HLA-DQmoleculesThe SPVL3antibodyhas beenusedin studiesof inhibition of antigen-specific cytolytic T cell clones.