2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

Granzyme B

Granzyme B (GrB) is a single chain and single domain serine protease. It is a member of the chymotrypsin superfamily. The process of GrB glycosylation results in generation of a 32 kDa and a 35 kDa glycosylated forms of GrB. The 32 kDa GrB forms contain high mannose oligosaccharide moieties and accumulate in cytotoxic T lymphocytes (CTLs) after T cell receptor (TCR) stimulation. In contrast, the 35 kDa GrB forms, which possess only the complex oligosaccharide groups, are not stored in CTLs and instead they are secreted through the constitutive calcium-independent secretory pathway after TCR activation. GrB is the most abundant serine protease stored in secretory granules of CTLs and NK cells. GrB can be produced by plasmacytoid dendritic cells (pecs). GrB-induced cell death is a primary mechanism in cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells to eliminate harmful target cells including allogeneic, virally infected and tumor cells. This mechanism implies activation of several pro-apoptotic pathways by direct proteolysis. The mannose 6-phosphate receptor has been identified as the plasma membrane receptor for GrB.

Lysed normal whole blood sample.

Clone: GB11 (IgG1 Mouse) GB11 cross reacts with: Mouse, Rat.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC

B46038

1 mL

Liquid

ASR

IOTest

cGMP

Helios

Helios belongs to the Ikaros transcription factor family. Members of this family are DNA binding proteins containing two-zinc finger N terminal domains and protein binding domain. Helios expression is restricted to the T lymphocytes lineage. Helios is suggested to play an important role in CD4+CD25+FoxP3+ regulatory cells. It has been shown that Helios up-regulates the expression of FoxP3 expression by binding to the FoxP3 promoter. Helios is a highly conserved transcription factor that has 97% sequence homology between human and mice.

Lysed normal whole blood sample.

Clone: 22F6 (IgG Hamster) The clone 22F6 reacts with human and mouse Helios.

Form

Format

Part#

Status

Line

Quality Standard

Size

PB

B49311

50 tests

Liquid

RUO

IOTest

cGMP

HIV-1 core antigen

Clone: KC57 (IgG1 Mouse) TheKC57antibodyidentifiesthe55, 39, 33 and24 kDaproteinsof thecoreantigensof thehumanimmunodeficiencyvirustype1 (HIV-1).The55 kDaproteinis the precursor protein for the core antigen. The 39 and 33 kDa proteins are intermediate products and the 24 kDa protein is the core protein.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC

6604665 6604667

100 tests 100 tests

Liquid Liquid

RUO RUO

cGMP cGMP

COULTER CLONE

RD1

COULTER CLONE

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 114 Outside US Flow Cytometry Catalog