2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

The CD279 antigen, also known as Programmed Death 1 (PD-1), belongs to the immunoglobulin superfamily. It contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic tail. It is encoded by the PDCD1 gene, which is located on the human chromosome 2q37.3. Immune cell function implicates important modulators known as cosignaling receptors. On T cells, the cosignaling receptors belong to either the immunoglobulin (CD28-like) or TNF receptor (TNFR) superfamilies. The CD28 immunoglobulin-superfamily is composed of coinhibitory and costimulatory receptors. Programmed Death-1 (PD-1) is one of the coinhibitory receptors, with notably Cytotoxic T Lymphocyte Antigen 4 (CTLA4) and CD272 (BTLA). The interaction of PD-1 with its ligands, PD-L1 and PD-L2, plays a critical role in regulating T cell activation and tolerance. CD279 ( PD-1 )

Lysed normal whole blood sample.

Clone: PD1.3 (IgG2b Mouse) The PD1.3monoclonalantibodyrecognizesT cells, activatedCD4 and CD8 positivecells and activatedB cells. This antibodyhas been assignedto the CD279 cluster of differentiation during the 8th HLDA Workshop on Human Leukocyte Differentiation Antigens, held in Adelaide, Australia, in 2004.

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

B30634 B36123 A78885 B30633

1 mL

Liquid Liquid Liquid Liquid

ASR ASR ASR ASR

IOTest IOTest IOTest IOTest

cGMP cGMP cGMP cGMP

PC5.5

0.5 mL 0.5 mL 0.5 mL

PC7 APC

CD294, also known as CRTH2, is a seven-transmembrane G-protein-coupled receptor known as the chemoattractant receptor-homologous molecule expressed on Th2 cells. Prostaglandin D receptor (DP), and CRTH2 have been identified as receptors for Prostaglandin D2 (PGD2), but they differ in their signaling pathways. PGD2 is the major metabolite of arachidonic acid produced by allergen-activated mast cells. CRTH2 molecule is preferentially expressed on human T-helper (Th) 2 and T cytotoxic (Tc) 2 cells but not on Th1 and Tc1 cells. CRTH2 is the most reliable surface marker selectively associated with circulating T (Th and Tc) cells able to produce IL-4 (as well as IL-5 and IL-13) and not IFN γ . On normal whole blood leucocytes, CD294 is also highly expressed on basophils and eosinophils. The expression of CD294 in peripheral blood mononuclear cells (PBMCs) is restricted to an activated state of Type 2 cells. CD294 may be weakly expressed on some monocytes and/or dendritic cells (CD14dim,CD16pos,HLA-DRpos,CD33pos). CD294 may act in mediating the recruitment and/or activation of basophils, eosinophils and Th2 cells at sites containing mast cells activated by invading allergens. CRTH2, but not DP, induces migration of Th2 cells, eosinophils and basophils in response to PGD2. CD294 ( CRTH2 )

Lysed normal whole blood sample. Gating on CD3 negative leukocytes.

Clone: BM16 (IgG2a Rat) The BM16 monoclonal antibody precipitates a 55 to 70 kDa protein from cell lysates of CRTH2-transfected Jurkatt and Th2 clone, corresponding to PGD2 receptor.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC

B42020 A07413

1 mL

Liquid Liquid

ASR RUO

IOTest IOTest

cGMP cGMP

PE

100 tests

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 105 Outside US Flow Cytometry Catalog