2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

The CD274 antigen is known as Programmed Cell Death 1 ligand 1 (PDCD1LG1 or PD- L1) or B7-H1. It is encoded by a gene located on the human chromosome 9p24. Immune cell function implicates important modulators known as cosignaling receptors. On T cells, the cosignaling receptors belong to either the immunoglobulin (CD28-like) or TNF receptor (TNFR) superfamilies. The CD28 immunoglobulin- superfamily is composed of coinhibitory (CD28, ICOS) and costimulatory receptors. Programmed Death- 1 (PD-1) is one of the three coinhibitory receptors with Cytotoxic T Lymphocyte Antigen 4 (CTLA4) and CD272. CD274 antigen is one of the two ligands, with PD-1 ligand 2 (PD-L2), for PD-1 and belongs to the B7 family. The PD-1 - PD-L1 interaction plays a critical role in regulating T cell activation and tolerance. In particular, it plays a role during development by inhibiting the TCR-mediated positive selection of thymocytes. It has also been shown to be implicated in the regulation of CD4 positive T cells proliferation. CD274 antigen is expressed on immature dendritic cells (iDC), mature dendritic cells (mDC), IFN- γ -treated monocytes, and follicular dendritic cells. CD274 ( PD-L1 )

Activated PBL 72h PHA.

Clone: PD-L1 (IgG1 Mouse) The PD-L1monoclonalantibodyrecognizesdendriticcells,T cellsand folliculardendriticcells.It has beenassignedto the CD274clusterof differentiationduring the 8th HLDA Workshop on Human Leukocyte Differentiation Antigens, held in Adelaide, Australia, in 2004.

Form

Format

Part#

Status

Line

Quality Standard

Size

PC7 APC

A78884 B36126

50 tests 0.5 mL

Liquid Liquid

RUO ASR

IOTest IOTest

cGMP cGMP

The CD278 antigen, also known as ICOS molecule (inducible T-cell costimulator, Inducible Costimulator), is a disulfide-linked homodimeric T-cell surface glycoprotein of 55-60 kDa, which belongs to the CD28 and CTLA-4 cell-surface receptor family. The CD278 molecule plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. While CD278 antigen is not constitutively expressed on naïve peripheral blood T cells, it is highly expressed on unstimulated thymocytes of germinal centers, showing a role in T cell differentiation. Upon activation by antigen presenting cells, T cells (CD4 positive cells and CD8 positive cells) express inducible co-stimulatory receptors, including CD28, CTLA4 and ICOS. The resulting interaction of CD278 (ICOS) with its ligand ICOS-L (B7-like molecule present on B cells) participates to interleukin production, especially IL-10 and IL4. The central role of CD278 in the generation and maintenance of humoral immunity has also been shown to be important. Upon the co-stimulation of T cells through ICOS and CD28, ICOS binds its ligand ICOS-L leading to IgG and IgM secretion as well as IgE production. It is not expressed on resting peripheral T cells, B cells, NK cells, monocytes/macrophages, platelets and granulocytes. CD278 ( ICOS )

Lysed normal whole blood sample.

Clone: ISA-3 (IgG1 Mouse)

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

A49080 B36127

100 tests

Liquid Liquid

RUO ASR

IOTest IOTest

cGMP cGMP

APC

0.5 mL

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 104 Outside US Flow Cytometry Catalog