2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

The CD209 antigen, also named dendritic cell (DC)-specific-intracellular adhesion molecule 3 (ICAM-3)-grabbing non-integrin (DC-SIGN), is a 44 kDa type II transmembrane C-type lectin with a single carbohydrate recognition domain (CRD). Like other C-type lectin members, CD209's extracellular structure is able to bind mannose residues in a Ca++ dependent manner. Highly specific to DCs, CD209 plays a key role in the primary immune response as receptor / processor for a variety of ligands, including: ICAM-3: initiation of DC-T cell immunological synapse; ICAM-2: transendothelial DC migration; HIV-1 gp120: capture of HIV-1 and transpresentation to CD4+ T cells; CMV envelope glycoprotein B: capture of CMV; Unknown ligands: capture of Ebola virus, and of Leishmania amastigotes. CD209 is expressed by immature DCs in peripheral tissues, with the noticable exception of Langerhans cells. It is also expressed by DCs in lymphoid tissues, and by certain macrophages. In peripheral blood, a percentage of plasmacytoid DCs (or DC2) may also express CD209. It is totally absent from monocytes, and an observed up-regulation of CD209 expression on monocyte-derived DCs correlates with the down-regulation of CD14. Monocyte-derived macrophages may express CD209, only when cultured in the presence of IL-13. CD209 ( DC-SIGN )

K562-SIGN Cell line.

Clone: AZND1 (IgG1 Mouse) The AZND1monoclonalantibody(mAb)is usefulfor manypurposes,includingflowcytometricanalysisof CD209-expressingcells,immunohistochemicalnalysis of CD209 + cells on frozentissuesections,immunoprecipationof CD209antigen,and investigationof its functions,due to the blockingor inhibitingpropertiesof AZND1 mAb. The AZND1 mAb to DC-SIGN has been assigned to the CD209 cluster of differentiation during HLDA 7.

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

A07407

100 tests

Liquid

RUO

IOTest

cGMP

The CD235a antigen, also called Glycophorin A, is a transmembrane, single-pass glycoprotein that contains carbohydrate, mostly in the form of sialic acid. CD235a has a molecular weight of 10 kDa. It is expressed by erythroid precursors and erythrocytes. Glycophorins A carries the antigen determinants for the MNS blood groups and has been proposed to be an inhibitor of hemagglutination and hemolysis. Glycophorin A binds siglec 5, and may also serves as receptor for cytokines and pathogens, including the malaria parasite, Plasmodium falciparum and some viruses, including influenza virus and hepatitis A virus. CD235a ( Glycophorin A )

HEL Cell line.

Clone: 11E4B-7-6 (IgG1 Mouse) The 11E4B-7-6(KC16) antibodybinds to the amino acid sequence27-39 of glycophorinA and has no reactivityto glycophorinB. It reacts with immature erythrocytes including erythroblasts, proerythroblasts and reticulocytes.

Form

Format

Part#

Status

Line

Quality Standard

Size

FITC FITC

B49206 IM2212U A07792 IM2211U A71564 A89314

100 tests

Liquid Liquid Liquid Liquid Liquid Liquid

CE/IVD

IOTest IOTest IOTest IOTest IOTest IOTest

98/79/EC

2 mL

ASR

cGMP

PE PE

100 tests

CE/IVD

98/79/EC

2 mL 1 mL

ASR ASR ASR

cGMP cGMP cGMP

PC7

APC-A750

0.5 mL

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 102 Outside US Flow Cytometry Catalog