2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

CD207 (Langerin) is an antigen described as selectively expressed by Langerhans cells (LC), a subset of immature dendritic cells (DC) formed in epidermis and mucosa. Langerin is a 40 kDa glycoprotein predominantly N-glycosylated which contains no disulfide bond. Its expression, restricted to the Langerhans cell lineage, is demonstrated at both the surface membrane and in the cytoplasm. Several lines of evidence indicate that Langerin is a marker specific for the acquisition of the Langerhans cell phenotype. It is down-regulated beyond the LC maturation stage. Langerin is not involved in antigen delivery into the MHC class II pathway but may play a role in antigen capture. Langerin is present only in epidermis and airway epithelia. Langerin is not detected in purified DCs isolated from peripheral blood, lymph nodes or thymus. CD1a+ DCs derived from CD34+ cord blood cell cultures in the presence of GM-CSF and TNF- α express Langerin between day 8 and 12. DCs derived from peripheral blood monocytes cultured with GM-CSF and IL-4 do not express Langerin. CD207 ( Langerin )

NIH-LG.13 Cell line.

Clone: DCGM4 (IgG1 Mouse) The DCGM4antibodystainsexclusivelyimmatureDCs of the LC lineage.Both intracytoplasmicand surfacemembranestainingis observed.DCGM4acts as a ligandfor Langerin,triggeringrapidendocytosiswithin20 minutesat 37 o C. The antibodyhas beenusedin intracellularimmunofluorescenceanalyzedin confocal microscopy.TheDCGM4antibodyhas beenusedto determinethe differentiationof the CD1a + /CD11c + subsetof bloodDCsintoLCs,andin the characterizationof DCs infiltrating breast carcinoma tissue.

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

IM3577

2 mL

Liquid

ASR

IOTest

cGMP

The DC-LAMP antigen (70-90 kDa) is a new member of the LAMP (lysosome- associated membrane protein) family. As other LAMP members such as CD68, DC- LAMP is a type I integral membrane protein of 416 amino acid residues, highly glycosylated (7 N- and some O- glycosylation sites), with 90% of the molecule located in the lumen of lysosomes. DC-LAMP is specifically expressed by those mature dendritic cells (DC) located in T cell areas of lymphoid tissues, which are known as interdigitating dendritic cells (IDC). A direct correlation between the expression of DC-LAMP and that of other maturation markers such as CD86 has been established. DC-LAMP first appears in the MHC Class II compartment, suggesting an important contribution of DC-LAMP to the processing of exogenous antigens. CD208 ( DC-LAMP ) Clone: 104.G4 (IgG1 Mouse) In flowcytometry,the 104.G4antibodyshows intracytoplasmicstainingof DCsderivedeitherfromCD34

NIH-DC.35 cell line.

+ cell culturesactivatedwithTNF α

andCD40L,or from

monocyte cultures activated with TNF α , LPS and CD40L.

Form

Format

Part#

Status

Line

Quality Standard

Size

UNLB

IM3448

0.2 mg

Liquid

RUO

-

cGMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 101 Outside US Flow Cytometry Catalog